Plaque Assay

A cell monolayer is treated with a dilution of a GFP-expressing virus. The cells are then coated with agar, which limits the virus spread to only neighboring cells.After incubation, GFP+ circles of infected cellsare counted for virus titration.

https://www.biotek.com/applications/visual-abstracts/Plaque%20Assay%20Visual%20Abstract-final.mp4