Applied Biosystems PowerUp SYBR Green Master Mix

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Thermo Fisher Scientific

Applied Biosystems PowerUp SYBR Green Master Mix

Applied Biosystems PowerUp™ SYBR™ Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows. Coupled with user-supplied primer sets and template, PowerUp™ SYBR™ Green Master Mix is designed to amplifiy targets for accurate gene expression analysis.

PowerUp™ SYBR™ Green Master Mix features include:

A dual hot-start mechanism for excellent specificity
Highly reproducible CTs over a broad dynamic range
Inclusion of UDG to help prevent carryover contamination
Stability of pre-assembled reactions for up to 72 hours
Compatibility with most real-time qPCR instruments

Specificity is paramount in obtaining high-quality data in SYBR™ Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding. PowerUp™ SYBR™ Green Master Mix uses the Dual-Lock™ Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures. In an experiment evaluating 24 different primer sets, PowerUp™ SYBR™ Green Master Mix generated single melt curves 100% of the time, consistent with highly specific amplification.

Technical Specifications
Application Note

Applied Biosystems PowerUp™ SYBR™ Green Master Mix demonstrates excellent reproducibility over a wide dynamic range for a variety of targets tested. In addition, this master mix provides efficient amplification over 6 logs of sample input. Unlike competitor mixes that can exhibit inhibition at high cDNA inputs and low correlation coefficients over a dynamic range experiment, PowerUp™ SYBR™ Green Master Mix efficiency is maintained over the full dynamic range to help deliver maximal confidence in data quality.

The inclusion of uracil-DNA glycosylase (UDG) and dUTP in the PowerUp™ SYBR™ Green Master Mix enables the degradation of any previously amplified PCR products, helping prevent contamination of subsequent qPCR reactions and possible false positives.

The tight control of the Dual-Lock™ Taq DNA polymerase in PowerUp™ SYBR™ Green Master Mix enables reactions to be assembled up to 72 hours prior to cycling without impacting data. This consistency allows users to have confidence and flexibility in the use of PowerUP™ SYBR™ Green Master Mix across numerous workflow scenarios.

Technical information:

Detection Method: SYBR
Format: Tube
PCR Method: qPCR
Polymerase: Dual-Lock Taq DNA Polymerase
Reaction Speed: Fast or Standard
For Use With (Equipment): 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12K Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, StepOne™, StepOnePlus™, ViiA™ 7 System
Product Line: PowerUP™, SYBR™
Product Type: Real-Time PCR SYBR Master Mix
Shipping Condition: Wet Ice
For Use With (Application): Gene Expression
Concentration: 2X
Sample Type: DNA

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