BioSpec-nano Spectrophotometer

DNA/RNA/Protein Analysis - GDO Analysis

BioSpec-nano Life Sciences Spectrophotometer is a spectrophotometer suitable for performing concentration controls for DNA and RNA nucleic acid samples. Quantification of DNA and RNA can be performed quickly and simply with very small samples ranging from 1µL, improving analysis efficiency thanks to special functions such as automatic sample insertion and deletion, and easy-to-use operating software. Analysis can be performed with 1 µL (path length: 0.2 mm) or 2 µL (path length: 0.7 mm) samples.

Easy Drop-and-Start Measurement

Automatic sample mounting eliminates the need for arm raising and lowering, and the automatic wiping feature makes wiping of the measurement sample unnecessary, enabling measurement of one sample after another.


 

Excellent Reproducibility and Measurement Accuracy

High reproducibility is obtained even when measuring low concentration samples.
High correlation is achieved with the measurement values provided with the double beam spectrophotometer.

Stress-Free Analysis with Data Judgment Feature

When measuring substances like proteins that do not easily form droplets, deposit more than 1 – 2 µL of sample.
Automatic data judgment based on the OD800 measurement value eliminates the need for concern about inadequate measurement caused by a drop in volume or the inclusion of bubbles.





 

Automatic Optical Pathlength Setting & Automatic Wiping

From setting the optical pathlength to measurement to wiping away the sample, it’s all automatic.
Tedious operations, like raising and lowering the arm to set the pathlength and wiping off the sample with a piece of cloth, are no longer required. There is no need to worry about whether the sample has been thoroughly wiped away, or whether the measurement site might become scratched. 
 

Low Carryover Achieved with Automatic Wiping Feature

The automatic wiping function of the BioSpec-nano greatly minimizes sample carryover from one measurement to the next.
Below are the results of alternate measurement of a double stranded DNA sample and a blank  (TE buffer), , each measured 60 times with automatic one-time wiping between measurements. In order to determine the level of carryover from the measured DNA sample, measurement of the next blank was conducted without replacing the wiper during the analyses. The results indicated that carryover was extremely low, averaging a mere 0.13%.










 

• Measuring wavelength range 220-800 nm
• Spectrum bandwidth 3 nm
• Wavelength accuracy ±1 nm
• Pathlength 0.2 mm, 0.7 mm
• Photometric range 0 ile 1.5 Abs
• Photometric value unit OD (Optical Density) is absorbance converted with 10 mm pathlength
• Sample volumes;  1 µL min. (pathlength: 0.2 mm), 2 µL min. (pathlength: 0.7 mm)
• Xenon Lamp Light Source
• Holographic Grating Monochrometer.
• Photodiode Array Detector.
• Auto-wiping function.
• 3 second  Spectrum measuring time.
• Analysis modes; Simple nucleic acid quantitation, labeled nucleic acid quantitation, protein quantitation, labeled protein quantitation, photometric measurement
• Simple nucleic acid quantitation; Nucleic acid concentration (RNA, dsDNA, ssDNA, OligoDNA) calculation, OD ratio (OD260 / 280, OD260 / 230) calculation
• Labeled nucleic acid quantitation; Nucleic acid concentration (RNA, dsDNA, ssDNA, OligoDNA), nucleotide concentration calculation, Label concentration, labeling ratio calculation, OD ratio (OD260 / 280) calculation
• Default labels (Cy 3, Cy 5, Alexa Fluor 546, Alexa Fluor 647)
• Detailed view (displays the focused sample analysis results and spectrum)