CaptureSelect™ CH1-XL Affinity Matrix

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Thermo Fisher Scientific

CaptureSelect™ CH1-XL Affinity Matrix

Technical Specifications
Application Note

CaptureSelect CH1-XL Affinity Matrix is the improved version of CaptureSelect IgG-CH1 Affinity Matrix. The specificity is the same, but it features a wider elution range (pH 3.0–4.5), increased binding capacity, and improved stability at higher pH.

CaptureSelect CH1-XL Affinity Matrix recognizes the CH1 domain of human IgG antibodies, enabling purification of all subclasses (1, 2, 3, and 4) of human IgG and Fab fragments, independent of the light-chain isotype and source material.

CaptureSelect CH1-XL Affinity Matrix features include:
• Platform approach for purification of IgGs and their Fab fragments directly from complex source materials in a single step with high purity and yield
• Elution possible at pH 4.0–4.5 with 50 mM acetate buffer
• No co-purification of overproduced free light chain due to selectivity for the CH1 domain
• No binding to bovine antibodies, so presence of fetal bovine serum is not a problem
• Excellent pH stability up to 50 mM NaOH for limited exposure
• Excellent scalability
• Non-animal derived

Main Characteristics

Matrix: agarose-based, epoxide-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect CH1-XL affinity ligand
Ligand coupling method: epoxide coupling
Binding capacity: >15 mg/mL human Fab fragment depending on flow rate, column height, contact time. For humanized Fabs lower capacities can be observed.
Elution conditions: 100 mM glycine buffer, pH 3.0; 50 mM acetate buffer, pH 4.0–4.5
Flow rate: 50–200 cm/h (up to 2 bar)
Formulation buffer: 20% (v/v) ethanol

We have developed a unique portfolio of affinity resins that help you develop next-generation antibody therapeutics. These affinity resins can be used for clinical and commercial production and include:

• A platform solution for Fab production parts regardless of light chain type
• Scalable solutions for weak Protein A binding
antibodies and antibody fragments
• Affinity resins offering gentle elution conditions for Fc-fusion proteins and pH-sensitive IgGs
• A cost-effective Protein A resin to help reduce clinical production cost

In addition to affinity resins, we also offer product-specific ELISAs for use in analytical assays to quantify any ligands potentially leached from the column and conjugated ligands.

CaptureSelect in Biosimilars and Recombinant Proteins

CaptureSelect helps you develop affinity resins, biosimilars, bio-enhanced products and other types of recombinant proteins.
These affinity resins can be used for clinical and commercial production. CaptureSelect protein purification products, non-antibody biomolecules
Exhibits affinity and specificity for target proteins for efficient one-step purification.

Selectivity - high purity in one step; raw material independent
Mild elution conditions - biological, retention of target activity
Reducing process steps — helps reduce costs, allows higher throughput
Effective removal of HCP, DNA, virus - high selectivity in a single capture step

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