Attune™  NxT

• Reliable—no-clog instrument design with Intelligent Probe results in higher productivity, less down time
• Broad compatibility—compatible with many different plate formats, including 96-, 384-, and deep-well plates
• Consistent data—designed to provide minimal variation regardless of sampling method (tube vs. plate) and collection rate
• Mixing by aspiration—mixing of sample by aspiration instead of shaking ensures homogeneity of the sample and maintains cell viability
• Simple to use—experiment setup has never been this simple

 

Consistent Results Across All Wells and Across Plates

Configuring Attune Flow Cytometer automation with the Attune CytKick and CytKick Max Autosampler demonstrates consistent results regardless of whether the sample is delivered from a tube or from a plate.

 

Attune NxT Autosampler offers consistent results regardless of sampling method 

Lysed whole blood was stained for CD45, CD4, and CD8 markers, aliquotted into both a tube and a plate, and analyzed on an Attune NxT flow cytometer. Minimal variation was observed between the sampling modes.

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To determine consistency across wells and plates, lysed human whole blood was labeled with fluorophore-conjugated monoclonal antibodies for CD45, CD3, CD4, and CD8 targets. The labeled cells were fixed, then added in equal amounts to each well of two 96-well plates, and acquired on a 4-laser Attune NxT Flow Cytometer with Autosampler. Both percent positive (left) and concentration (events/µL, right) were highly consistent across wells for all three identified populations.

Consistency of percent positive across 192 Sample. Consistency of all 192 wells for analysis of percent positive of parent gate of the three identified populations.	Consistency of concentration across 192 Samples. Consistency of all 192 wells for analysis of concentration of the three identified populations.

Exact Results

The Attune NxT Autosampler was designed to minimize system down time. Automated sampling devices are often plagued by clogging or probe crashes. The Intelligent Probe, with a 200-micron diameter that is larger than most probes available in the industry, minimizes instances of clogging.

To prevent probe crashes, which may require expensive probe replacement, the Attune NxT Autosampler automatically performs a probe self-calibration every month to help ensure consistent positioning of the probe within every well. The self-calibration verifies proper function of the probe, reduces variability in probe positioning over time to prevent failures during plate runs, and helps improve reliability over the life of the system. Self-calibration of the probe also allows the autosampler to be easily moved, so it can be shared between multiple instruments.

Robotic Automation

Extend your unmanned runtime settings and scalability with robotic integration applications for the Attune Flow Cytometers. Our range of automation solutions include robotic plate taxiing, extended fluidics, temperature-stable plate storage, and software for operations. You can leverage both scheduling and integration to get the most from your solution.

 

Broad Compatibility

The Attune NxT Autosampler allows use of many different plate formats, including 96-, 384-, and deep-well plates, without being restricted to a single manufacturer. To maintain system flexibility, the manual sample probe is always accessible even when the loader is present, and switching between tubes and plates is as simple as a flip of a switch.

Consistent Data

Unlike other plate loaders that mix samples by shaking, which may not ensure homogeneity of the sample, the Attune NxT Autosampler mixes samples by aspiration, enabling uniform mixing while preserving sample viability and resulting in consistent data throughout the plate.

Simple to Use

The instrument design and software were developed with customer ease-of-use in mind. The Attune NxT Autosampler has fluidic bottles with sensors that inform the user when the fluids need to be replaced. The autosampler’s indicator lights and warning systems have been designed to ensure that a user who has walked away from the system can easily see the system status at all times.

Setting up an experiment with the Attune NxT cytometric software is simple. Using a plate layout view, users can create compensation wells, define instrument settings, and identify samples. The intuitive software allows quick learning and data analysis. For example, a heat map view allows rapid screening and confirmation of samples while the instrument is acquiring data.
 

Specifications
Capacity	1 x 96-well or 384-well plate (standard or deep-well)
Format	384-well plate, 96-well plate
For Use With (Equipment)	Attune™ Acoustic Focusing Cytometer
Product Type	Flow Cytometer Autosampler
Product Line	Attune™

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