Thermo Fisher Scientific

Invitrogen™ EVOS™ M7000 | Cellular Imaging System

Invitrogen™ EVOS™ M7000 microscopes are a fully automated, no-personal contact imaging system. It includes both monochrome and color high resolution CMOS cameras for the best of both fluorescent and colorimetric imaging. The M7000 Cell Imaging System can automatically scan multi-well plates and features fast autofocus, image acquisition and big data processing.
Designed by biologists for biologists, EVOS imaging systems incorporate high-resolution cameras, bright and digitally controlled LED light sources, and intuitive software to help you capture stunning, publication-quality images in just a few clicks. These fully automated, multichannel fluorescence or brightfield microscopes feature advanced imaging tools to handle time-lapse movies, multiwell plate scanning, image stitching and tiling, and cell counting.
 
 

Compact, Efficient Design

EVOS microscopes are designed with scientists’ workspace and workflow needs in mind. Components and controls are conveniently integrated into a single, lightweight system that fits on a benchtop and can easily be moved to classrooms, teaching labs, and conference rooms. The size and portablility of EVOS microscopes let you capture and view images when and where needed.

EVOS microscopes design make them apt fo classrooms, teaching labs and conference rooms














 

 

 

Bright LED Light Sources

All EVOS imaging systems feature bright LED light sources that provide more than 50,000 hours of brilliant (yet adjustable) illumination that (unlike mercury arc lamps) remains constant with minimal degradation over time. Cost-efficient and environmentally friendly, EVOS LED light cubes are designed for precise control, minimal maintenance, and exceptional reliability.

For the M7000 and M5000, a choice of compact, easily interchangeable LED light cubes can be matched to the spectra of specific dyes or fluorescent proteins in your application. Each cube incorporates excitation filter, dichroic beam-splitter, and emission filter. Filters are hard-coated for maximum transmission efficiency.



Five popular EVOS LED cubes version 2.0 for RFP, GFP, DAPI, CY5 and TxRed are shown











 

EVOS M7000 - Automated, secure, live-cell imaging, tiling and image compositing


 

EVOS M7000- Z-stacking, 2D/3D- deconvolution, visualization and analysis

EVOS M7000 - Optimized light cubes and software features

Constant Light Intensity 

Mercury arc lamps can lose 50% of their intensity in the first 100 hours of operation! In addition, images acquired in different sessions cannot be quantitatively compared using without complicated calibrations. Because EVOS LED light sources produce constant light intensity, users can rely on consistent illumination and can compare results quantitatively from images acquired on different days.














 

Less Expensive to Own and Maintain

The LED bulbs on the EVOS systems are rated for >50,000 hours (~17 years), compared to 300 hours for a typical mercury bulb (and 1,500 hours for a metal halide bulb). That means significant savings in the overall upkeep of your instrument.












 

 

Hard-coated Filter Sets for Higher Transmission Efficiency

Hard-coated filter sets are more expensive but have sharper edges and significantly higher transmission efficiencies that typically result in >25% more light transmission than traditional soft-coated filters. With the EVOS system’s hard-coated filter sets, your light cubes cost less over time—and provide brighter fluorescence, higher transmission efficiencies, the ability to detect faint fluorescence signals, and better signal-to-noise ratios.
























 

Stunning, Easy-to-Capture Images

The key feature shared by all EVOS cell imaging systems is exceptional image quality. Capture clear, bright, broadcast-quality images and videos to help tell the story of your data in just a few clicks.

 
Image shows HeLa cell labeled with Phalloidin-Alexa Fluor Plus 555 and NucBlue, image shows 2D deconvolution effect with Celleste software Image shows EVOS software tiles and stitches 13x18 images to create a spotless composite image
HeLa cell large field vs. resolved EVOS M7000, 60x objective, Celleste Image Analysis Software. Stains: NucBlue (core), Phalloidin. Complete suturing of live HeLa cells
EVOS M7000, 10x lens, 13x18 stitched image. Stain: Calcein AM. The resolution of the individual field of view is maintained when erecting an entire well.
   
Image shows HeLa cells labeled and then imaged with EVOS M7000, monochrome camera. Image shows rat intestine section cells labeled and then imaged with EVOS M7000, color camera.
HeLa cells
EVOS M7000, monochrome camera.
Cells proliferating in the animal gut part
EVOS M7000, color camera.
   
Image shows bovine pulmonary endothelial cells labeled for F-actin, microtubules and nucleus, and imaged with EVOS M7000 a group of red and green fish
Bovine pulmonary artery endothelial cells
EVOS M7000. Stains: Phalloidin (F-actin), BODIPY IgG (microtubules), DAPI (core).
Wound healing
EVOS M7000, 10x, Stage Incubator over 21 hours. Stains: DsRed-cadherin-4, GFP.
  

High Definition Cameras and Lens Options

Depending on the model, EVOS cell imaging systems are a high-sensitivity monochrome camera ideal for fluorescent imaging; high sensitivity color camera ideal for colorimetric imaging; or both monochrome and color cameras for maximum flexibility.

A choice of 30+ high-performance objective lenses ranging from 1.25–100x allow you to adjust the optics for imaging type, sample type and vessel. Image acquisition is fast and with automated multi-well scanning, the M7000 can scan a 96-well plate in less than 5 minutes across 3 fluorescent channels. EVOS cell imaging systems make it easy to capture broadcast quality images in seconds.
 

Powerful, Intuitive Software

All EVOS microscopes come with onboard software that allows you to view, capture, and process images quickly and easily. You can:

  • select colors and adjust brightness while viewing an image
  • adjust contrast, saturation, and color balance
  • choose between high resolution and quick capture
  • capture and save images

On the M7000 and M5000, the onboard software offers pinpoint operational control and powerful image processing tools such as cell counting, confluence, Z-stacking, image tiling and stitching, time-lapse movies, and RGB synthesis. These tools operate intuitively with minimal training, aided by onboard SmartStart guides. Autofocus and other automated tools save time and effort. The M7000 adds advanced automation features and complete programmatic control, allowing imaging through software alone, without ever touching the instrument. Download the most recent EVOS M7000 and M5000 software updates.

The software embedded in the M7000 and M5000 provides point operational control and powerful image processing tools such as cell counting, confluence, Z-stacking, image tiling and compositing, time-lapse movies, and RGB synthesis. These tools operate intuitively with minimal training with the help of built-in SmartStart guides. Autofocus and other automatic tools save time and effort. The M7000 adds advanced automation features and complete programmatic control, allowing for software-only viewing without ever touching the tool. Download the latest EVOS M7000 and M5000 software updates.

















 

Enhanced Safety, Minimal Handling

With all EVOS imaging systems, a large, bright, high-definition monitor lets you view your cells outside the confines of a darkroom and facilitates safe collaboration in nearly any space. There’s no need to remove safety goggles to look through an ocular eyepiece. This minimizes difficult inter-user decontamination and facilitates both collaboration and safety.

















The EVOS M5000, FLoid, and XL Core are designed for use in a biosafety cabinet or hood while wearing gowns, gloves, and goggles or face shields if necessary. There’s minimal risk of contamination when you passage, split, transfect, transduce, or otherwise manipulate your cells.









 

 

Thermo Fisher Cloud Connection

Images from all EVOS instruments can be sorted and analyzed with the EVOS Image Analysis app in Connect, Thermo Fisher’s secure, cloud-based platform for data storage, scientific analysis apps, and peer collaboration tools. Once uploaded, you and your colleagues can collaboratively access your gallery and retrieve, analyze, and manipulate your images from any web browser around the world. You can also run other Connect applications and monitor the status and configuration of your connected instruments.





















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