Qubit™ 4

• More sensitive than UV absorbance–based quantification, making it ideal for precious samples
• Accurately quantifies DNA, RNA, and protein in < 3 seconds per sample
• Fast, reliable detection of degraded RNA with the new RNA IQ assay
• New integrated reagent calculator reports amount of dye and buffer needed
• Uses as little as 1 μL of sample
• Flexible options for exporting results: Wi-Fi, USB drive, or direct connection with a USB cable

How the Qubit Fluorometer works
Single-sample measurements are easy

This user-friendly fluorometer delivers results using quick, simple assay procedures. The large, color touch screen allows you to easily navigate through the various options. Calculations and settings are automatically performed by the instrument. The Qubit 4 Fluorometer will save the data for up to 1,000 samples, and you can transfer data using Wi-Fi, a USB drive, or direct connection with a USB cable. In addition, new Qubit 4 includes a built-in reagent calculator to easily determine the amount of dye and buffer required to prepare your samples and standards.










 

Assays for use with the Qubit Fluorometer

The Qubit 4 Fluorometer and all Qubit assays are optimized for use together. Qubit assays are highly sensitive and selective for DNA, RNA or protein. These assays are performed using the same basic protocol: simply mix and read, with incubation times of only 2 minutes required for DNA and RNA assays or 15 minutes for the protein assay. Qubit Assays cover a broad dynamic range for any starting sample concentration. In addition to our core assays for dsDNA, oligos, total RNA, microRNA, and protein, Ion Sphere Particle quality can be evaluated on the Qubit 4 Fluorometer using the Ion Sphere Quality Control Kit prior to performing a sequencing run on the Ion Personal Genome Machine (PGM) Sequencer.

Comparison of sample concentration ranges for Qubit assays and UV absorbance measurements. 
UV absorbance readings are not selective for RNA vs DNA. The Qubit 4 Fluorometer and all Qubit assays
are optimized for use together. 

Sensitive, Accurate Biomolecule Quantification

The Qubit 4 Fluorometer detects fluorescent dyes that bind specifically to the target of interest. This target specific enables precise, accurate measurements even for dilute samples requiring high sensitivity.
 

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