Micro-matrix Bioreactors (1-10 ml)

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Micro-matrix Bioreactors (1-10 ml)

The Micro-Matrix allows control of up to 24 independent bioreactors in a single microtiter plate area.
For each of the 24 bioreactors with a volume of 1-10 ml on the plate, there is independent control of the following parameters, just like larger scale bioreactors:

• pH (measurement and double-sided control)
• temperature (measurement and two-sided control, possibility to create gradient across the whole plate)
• dissolved oxygen control (measurement and bilateral control)
• individual liquid addition (including feed profiles)
• up to 4 different gas additions, each individually controlled

Key Features

Minimum space requirement in laboratory
Monitoring and control of pH and dissolved oxygen
Separate control of wells
Parallel operation of 24 bioreactors

Application Areas

Screening of cell line, microbial or yeast libraries,
Process development studies,
Process optimization studies,
Small volume culture studies.

Technical Specifications
Application Note

The Micro-Matrix allows control of up to 24 independent bioreactors in a single microtiter plate area.

For each of the 24 bioreactors with a volume of 1-10 ml on the plate, there is independent control of the following parameters, just like larger scale bioreactors:

pH (measurement and two-sided control)
temperature (measurement and two-sided control, possibility to create gradient across the whole plate)
dissolved oxygen control (measurement and two-sided control)
individual liquid addition (including feed profiles)
Up to 4 different gas additions, each individually controlled

Software

Tape

Each well on the cassette has a separate pH and oxygen sensor and each well has 3 temperature sensors mounted under the cassette, guaranteeing optimum temperature measurement and control.

General:

Number of wells per cassette 24
Total volume per bioreactor well 10 mL
Working volume per bioreactor well 1 - 5 mL

Mixing:

Orbit radius 25 mm diameter
RPM range 0 - 400 RPM
Mixing times < 1 sec

Aeration:

Gas delivery 0.1 - 18 mL/min Per gas, per well
Number of gasses 4
Gas feed pressure > 1.0 (extern)

Liquid additions:

Liquid delivery > 50 nl
Number of liquids is 1 per 6 wells
Liquid feed pressure > 2.0 bar (extern)

Temperature:

Measurement range 0 - 45 °C
Resolution 0.1 °C
Accuracy 0.5 °C
Temperature control range 15 - 45 °C
∆T between adjacent wells 1 °C
Heat-up time 1 °C / min 20 °C to 37 °C
Control accuracy ± 0.1 °C

pH:

Measurement 5.5 - 8.5 pH
Resolution < 0.05 pH @ pH 7.2
Accuracy < 0.1 pH @ pH 7.2
pH control range 5.5 - 8.5 pH
Control accuracy ± 0.1 pH

Dissolved Oxygen:

Measurement 0 - 150 % Air saturation
Resolution < 1 % Air saturation
Accuracy < 0.5% @ 0% air saturation
< 3% @ 100% air saturation
DO control range 0 - 150 %
Control accuracy ± 5% Air saturation
Oxygen Transfer Rate 250 mmol / L / h

Dimensions:

415 mm Height
570 mm Depth
560 mm Width

Weight:

65 kg

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