Applied Biosystems QuantStudio Absolute Q Dijital PCR System

Adding Offer List

Thermo Fisher Scientific

Applied Biosystems QuantStudio Absolute Q Dijital PCR System

The Applied Biosystems QuantStudio Absolute Q Digital PCR System is a plate-based digital PCR (dPCR) platform powered by proprietary microfluidic array plate (MAP) technology. that enables all the necessary steps for dPCR—compartmentalizing, thermal cycling, and data acquisition—to be conducted on a single instrument. The dPCR workflow is identical to the qPCR workflow you are familiar with to improve ease of use, minimize hands on steps, and maximize consistency.

dPCR overcomes variability and low accuracy by eliminating the need for a standard curve. The QuantStudio Absolute Q Digital PCR System consists of just one instrument, consolidating all steps required into a single plate and transforming a multi-step multi-instrument workflow into a one-step qPCR-like workflow.

Technical Specifications
Application Note

The QuantStudio Absolute Q Digital PCR System relies on its proprietary microfluidic array plate (MAP) technology to deliver highly-accurate dPCR results. The MAP has 16 dPCR reaction units, easily distinguished as opaque squares. Zooming in, each dPCR reaction unit (opaque square) is made up of 20,480 fixed array microchambers. The microchambers themselves are connected by a distribution network that is used to deliver PCR reagents. Once the reagents have been compartmentalized into the microchambers, PCR amplification then proceeds, and the number of microchambers with successful DNA amplification are counted.

Dye compatibility	See Optical Configuration for additional details. FAM, HEX, VIC, ABY, ROX, CY5, JUN
Optical multiplexing	Four optical chanels available (five optical channel system, ROX used for reference)
Dynamic range	Five logs
Sensitivity	1–100,000 copies
Precision	±10%
Sample throughput	Up to 16 samples per run. MAP16 plate can be run in intervals of four (i.e., 4, 8, 12, or 16)
Micro-chambers per reaction	20,480
Percentage sample analyzed	95%
Dimensions (unpacked)		620 mm (l) x 600 mm (w) x 540 mm (h)  24.5 (l) x 23.5 (w) x 21 .2 (h) in
Dimensions (packaged)		826 (l) x 940 (w) x 899 (h) mm 32.5(l) x 37(w) x 35(h) in
Weight		Approx. 60 kg
Connections		Power, USB 3.0 (to dedicated PC)
Cooling mode		Forced convection
Illumination		Rax, blue, phosphor green high-power LED
Optical channels		Five (fixed configuration)
Power input		100–240 Vac, 50–60Hz

Optical Configuration

#	Color	Excitation filter peak λ	Emission filter peak λ	Examples of compatible dyes
1	Blue	466	520	FAM
2	Green	514	560	HEX  VIC
3	Yellow	549	589	ABY
4	Red	589	625	ROX
5	Dark Red	630	684	JUN  CY5

Send Me Application Note

Recommend Me a System

First Name *

Last Name *

Organization *

Phone *

E-mail *

City *

Sample Name / Type *

Which compounds/parameters will you analyse *

Detection limit *

Notes

By submitting your information, you agree that AntTeknik may collect and further process your personal data for marketing purposes and in accordance with our Privacy Policy.

Please Recommend Me a Column