Applied Biosystems StepOnePlus™ Real-Time PCR System

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Thermo Fisher Scientific

Applied Biosystems StepOnePlus™ Real-Time PCR System

Applied Biosystems StepOnePlus™ Real-Time PCR System is a 96-well Real-Time PCR instrument perfect for both first-time and experienced users. The StepOnePlus™ Real-Time PCR System can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software. Utilizing robust LED based 4-color optical recording, the StepOnePlus™ Real-Time PCR System is designed to deliver precise, quantitative Real-Time PCR results for a variety of genomic research applications.

This remarkably simple Real-Time PCR system is designed with an LCD touchscreen and a user-friendly interface that brings the power of genetic studies to researchers new to Real-Time PCR. The intuitive software and protocol wizards help guide new users through their Real-Time PCR experiments. The StepOnePlus™ Real-Time PCR System also includes a quick-start setup so that you start a run immediately and enter plate information at a later time.

Technical Specifications
Application Note

Applied Biosystems StepOnePlus™ Real-Time PCR System utilizes a long-life LED-based optical system that can record fluorescence from FAM™/SYBR® Green, VIC®/JOE™, NED™/TAMRA™, and ROX™ dyes. This cost-effective, 4-color, 96-well system delivers precise, quantitative Real-Time PCR results and saves data from all filters in every run without depending on a computer or plate setup. It can discriminate between 2 populations of 5,000 and 10,000 template copies of a TaqMan® assay with 99.7% confidence.

The StepOnePlus™ System supports many Real-Time PCR applications:
• SNP Genotyping
• Gene Expression Analysis
• MicroRNA Expression
• Protein Expression
• Translocation Analysis
• Gene Detection
• Viral Load Analysis

The included StepOne™ Software supports a variety of analysis methods:

• Standard Curve (absolute quantitation)
• Relative Standard Curve
• Comparative Ct (ΔΔCt) (relative quantitation)
• Genotyping and Presence/Absence
• Melt Curve Analysis (as a standalone application)
• High Resolution Melting (as a standalone application)

Specifications

Format: 96-well Plate, 8-tube Strips, 0.1 ml Tubes
Thermal Accuracy: 0.25°C (35°C to 95°C) of Display Temperature
Sample Ramp Rate: Fast Mode: ± 2.2°C⁄s, Standard mode: ± 1.6°C⁄s
Sensitivity: 1 Copy
Temperature Range (Metric): 4 to 100°C
Reaction Volume Range: 10-30 µl (Standard Curve Experiments: 40 µl in Standard Mode is Validated)
Passive Reference Dye: ROX (Separate Tube), No ROX, ROX (Pre-mixed)
Dynamic Range: Linear Dynamic Range greater than 9 Log Units (Detection)
Calibrated Dye: VIC™, SYBR™ Green I, ROX™, JOE™, NED, FAM™, TAMRA™
High-throughput Compatibility: Multiplexing
For Use With (Equipment): StepOnePlus™
Thermal Uniformity: 0.25°C (35°C to 95°C) of Setpoint/Display Temperature
Reaction Speed: Fast, Standard
Detection Method: SYBR, Primer-Probe Detection
Run Time: <2 hrs⁄run (Standard Mode), <40 min⁄run (Fast Mode)
Optics: 4 Emission Filters, Blue LED Excitation source, photodiode
Thermal Cycling System: Peltier-based System
Dimensions: 24.6 cm/9.7 in.(W) x 42.7 cm/16.8 in.(D) x 51.2 cm/20.2 in.(H)
Capacity: 96 x 0.1 ml Tubes, 12 x 8-tube Strips, 1 x 96-well Plate
Product Line: StepOnePlus™
Peak Block Ramp Rate: 4.6°C⁄s
Display Type: LCD Touchscreen
Green Features: Sustainable Packaging
Weight: 24 kg

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