Applied Biosystems TaqMan Fast Universal Master Mix

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Thermo Fisher Scientific

Applied Biosystems TaqMan Fast Universal Master Mix

Applied Biosystems TaqMan Fast Universal Master Mix (2X), No AmpErase UNG, delivers results in 40 minutes for 40 cycles of PCR in a 20-µL reaction volume using the Applied Biosystems 7900HT and Fast 7500 Fast real-time PCR systems. The optimized formulation contains AmpliTaq Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and allowing room temperature reaction setup. Additionally, a proprietary ROX dye serves as a passive internal reference to normalize non-PCR-related fluorescence fluctuations, for superb precision on Applied Biosystems real-time PCR instruments.

Technical Specifications
Application Note

Technical information:

Detection Method: Primer-Probe
Format: Tube
GC-Rich PCR Performance: High
PCR Method: qPCR
Polymerase: AmpliTaq DNA Polymerase
Reaction Speed: Fast
Passive Reference Dye: ROX (Pre-mixed)
Product Line: AmpErase™, TaqMan™
Product Type: Fast Universal PCR Master Mix
For Use With (Application): Gene Expression, Genotyping, miRNA analysis
Concentration: 2X
Fidelity (vs. Taq): 2X
Sample Type: DNA (Genomic), cDNA

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